W-X
Western Blot Transfer Buffer
-See Transfer Buffer
Western Coomassie Blue Stain (for Nitrocellulose membrane staining)
5 g Coomassie Brilliant blue R250 (0.25% final)
140 ml Glacial Acetic Acid (7% final)
1000 ml methanol (50% final)
855 ml dH20
Western (0.5 M Tris Base) Western (2 M Tris Base)
Tris Base 60.55 g 242.2 g
dH20 800 ml 800 ml
pH to 6.8 pH to 8.8
QS to 1 L; filter.
Wash Buffer (8X, Nickel Column)
100 ml 200 ml
480 mM IDA 3.3 g 6.6 g
4 M NaCl 23.4 g 46.8 g
160 mM Tris-HCl, pH 8.0 16 ml (1M stock) 32 ml (1M stock)
QS to vol.
Filter and store at 4°C
100 ml 200 ml
480 mM IDA 3.3 g 6.6 g
4 M NaCl 23.4 g 46.8 g
160 mM Tris-HCl, pH 8.0 16 ml (1M stock) 32 ml (1M stock)
QS to vol.
Filter and store at 4°C